| & | SoCon | 3:14.40 | Western Carolina | ||
| $ | CPTF | 3:12.83 | Arp, Bowman, Dailey, Brown | Shippensburg |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | A | Rodriguez | Thoma | Adams | Clayton | NT | ||
| 5 | WCU |
A | Miles | Haley, Jr | Manigault | Smith | 3:32.67 | Buccaneer T&F Invitational |
| 6 | FURM |
A | Epstein | Ogden | Coppi | Powers | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | A | Parrilla | Mills | Turnbow | Walsh | 3:25.62 | VMI Team Challenge | |
| 3 | CIT |
A | Duncan | Newton | Shipley | Manon | 3:22.97 | Carolina Indoor Open |
| 4 | WOFF |
A | Larkin III | Ellis | Murphy | Bull | 3:18.66 | Darius Dixon Memorial Invite 2021 |
| 5 | SAMF |
A | Diggs | Peñalver | McMahan | Fitzpatrick | 3:16.85 | Jaguar Invitational |
| 6 | VMI |
A | Gray | Whittaker | Poindexter | Walker | 3:18.63 | Winter Classic 2021 |