| & | SoCon | 3:44.98 | Western Carolina | ||
| $ | CPTF | 3:46.45 | Runge, Maisto, Johnson, Swierbut | William and Mary |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | FURM |
A | Marvin | Kuntz | Trone | Nguyen | NT | |
| 5 | CIT |
A | Royer | Jackson | Murray | Mitchell | 4:11.82 | VMI Winter Relays 2021 |
| 6 | CHAT |
A | Williamson | Broling | Davis | Bryant | 4:28.66 | Samford January Invite 2021 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | WCU |
A | Delisser | Robinson | Blakely | McIntyre | 4:00.48 | Buccaneer T&F Invitational |
| 3 | A | Cooper | Johnson | Lord | Eliscar | 3:58.35 | Darius Dixon Memorial Invite 2021 | |
| 4 | SAMF |
A | Lueders | Cole | Strozier | Dumar | 3:54.51 | UAB Vulcan Invitational |
| 5 | VMI |
A | Ruffin | Parson | Linkous | Williams | 3:53.52 | Winter Classic 2021 |
| 6 | WOFF |
A | Cutler | McNinch | Jefferson | McDevitt | 3:54.01 | Darius Dixon Memorial Invite 2021 |